The Rafah crossing on Gaza's border with Egypt closed on Saturday for Coptic Christmas, said the Hamas-controlled Ministry of Interior in Gaza.
The terminal will reopen Sunday, the ministry said in a statement.
The Rafah crossing closes on all Egyptian public holidays, as part of an agreement between the governments in Gaza and Cairo.
Rafah is one of two crossings through which Palestinians can exit Gaza; the other is controlled by Israel. Both crossings were closed as part of an embargo policy by Egypt during the rule of former President Hosni Mubarak, as Israel aimed at cutting off Hamas.
Since 28 May, Egypt has applied a new system for the Rafah border crossing, in which the terminal is being opened permanently and only closed on the weekends.
According to formal Egyptian figures, the number of people having entered Egypt since 28 May through the Rafah crossing has reached more than 210,190.